GSM5503255: HNF4A input PP1 WT 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
HNF4A
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived pancreatic cells
NA
NA
Attributes by original data submitter
Sample
source_name
ES-derived progenitors
differentiation stages
Early pancreatic progenitor
genotype
wild type
antibody
HNF4A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked by formaldehyde and lysated by SDS lysis buffer. Sonicated nuclei were incubated with antibody overnight and then pulled down by dynabeads. After washing, decrosslinking was performed to isolate DNA. Samples were amplified using NEBNext Q5 Hot Start HiFi PCR Master Mix. The concentration of samples was checked using PicoGreen and median fragment size was checked using the Agilent D1000 screentape on the Agilent Technologies 2200 TapeStation. Samples were sequenced single end or paired, as specified, on Illumina HiSeq 2500.