Antigen

Antigen Class

TFs and others

Antigen

HNF4A

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived pancreatic cells

NA

NA

Sample

source_name

ES-derived progenitors

differentiation stages

Early pancreatic progenitor

genotype

wild type

antibody

HNF4A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked by formaldehyde and lysated by SDS lysis buffer. Sonicated nuclei were incubated with antibody overnight and then pulled down by dynabeads. After washing, decrosslinking was performed to isolate DNA. Samples were amplified using NEBNext Q5 Hot Start HiFi PCR Master Mix. The concentration of samples was checked using PicoGreen and median fragment size was checked using the Agilent D1000 screentape on the Agilent Technologies 2200 TapeStation. Samples were sequenced single end or paired, as specified, on Illumina HiSeq 2500.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

15412262

Reads aligned (%)

92.5

Duplicates removed (%)

1.4

Number of peaks

412 (qval < 1E-05)

hg19

Number of total reads

15412262

Reads aligned (%)

91.5

Duplicates removed (%)

1.6

Number of peaks

366 (qval < 1E-05)